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e2621 experimental models  (New England Biolabs)


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    Structured Review

    New England Biolabs e2621 experimental models
    E2621 Experimental Models, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2621+experimental+models/NEBuilder+HiFi+DNA+Assembly+Master+Mix/pm41344332-264-213-209
    Average 99 stars, based on 9183 article reviews
    e2621 experimental models - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Virus:

    Article Title: Targeted inhibition of mu-opioid receptors in neuronal subpopulations by membrane-tethered Naloxo-DART antagonists.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9-pDISPLAY-Halo-LK3-TM Virovek, this paper N/A pAAV9-Ef1a-DIO-EYFP Gift from Karl Deisseroth Addgene viral prep Cat# 27056-AAV9 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 11965092 Fetal Bovine Serum (FBS) Gibco Cat# A5256701 Earle’s balanced salt solution (EBSS) Life Technologies Cat# 14155063 Polyethylenimine (PEI) PolySciences Inc Cat# 23966 Penicillin-Streptomycin Gibco Cat# 15140122 D-PBS Gibco Cat# 14190144 DAMGO, [ 3 H] Novandi Chemistry AB Cat# NT1077 coelenterazine h NanoLight Cat# 301 Poly-D-Lysine hydrobromide Sigma Aldrich Cat# P6407 Nano-Glo luciferase assay system (Furimizine) Promega Cat# N1130 [D-Ala 2 , N-MePhe 4 , Gly-ol]-enkephalin (DAMGO) Tocris Biosciences Cat# 1171 SNC80 Tocris Biosciences Cat# 0764/10 (− )-U50488 Tocris Biosciences Cat# 0496/10 nociceptin Abcam Cat# ab120741 SNAP-Surface® Alexa Fluor® 488 New England Biolabs Cat# S9124S HaloTag® Alexa Fluor® 660 Promega Cat# G1001 ketamine Sigma Aldrich Cat# K113 xylazine Sigma Aldrich Cat# K113 isoflurane Zoetis UK Limited Cat# 50019100 Lumafluor Retrobeads Lumafluor Inc. N/A Carprofen (Rimadyl) Zoetis UK Limited N/A Amoxicillin (Betamox) Norbrook UK N/A Naloxone NIDA N/A SulfoCy5-buprenorphine University of Nottingham, Schembri et al. 27 N/A Lumi4-Tb Revvity Cat# SSNPTBD Tag-lite SNAP-CLIP labeling reagent (5x) Revvity Cat# LABMED Critical commercial assays Bradford protein assay Bio-Rad, Hercules, CA Cat# 5000001 Gibson HiFi assembly kit New England Biolabs Cat# E2621 Experimental models: cell lines HEK 293T (Female) ATCC (Virginia, USA) Cat# CRL-3216 Flp-InTM-CHO Cell Line InvitrogenTM/ThermoFisher Scientific Cat# R75807 Experimental models: organisms/strains B6.Cg-7630403G23Rik Tg(Th− cre)1Tmd /J TH-Cre1 mice Jackson Laboratory, Bar Harbor, Maine Strain #008601 RRID:IMSR_JAX:008601 Recombinant DNA WT-Gαi 2 cDNA Resource Center, cDNA.org Cat# GNAI200000 masGRKct-Rluc8 Nevin Lambert, University of Georgia. ..

    Recombinant:

    Article Title: Targeted inhibition of mu-opioid receptors in neuronal subpopulations by membrane-tethered Naloxo-DART antagonists.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9-pDISPLAY-Halo-LK3-TM Virovek, this paper N/A pAAV9-Ef1a-DIO-EYFP Gift from Karl Deisseroth Addgene viral prep Cat# 27056-AAV9 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 11965092 Fetal Bovine Serum (FBS) Gibco Cat# A5256701 Earle’s balanced salt solution (EBSS) Life Technologies Cat# 14155063 Polyethylenimine (PEI) PolySciences Inc Cat# 23966 Penicillin-Streptomycin Gibco Cat# 15140122 D-PBS Gibco Cat# 14190144 DAMGO, [ 3 H] Novandi Chemistry AB Cat# NT1077 coelenterazine h NanoLight Cat# 301 Poly-D-Lysine hydrobromide Sigma Aldrich Cat# P6407 Nano-Glo luciferase assay system (Furimizine) Promega Cat# N1130 [D-Ala 2 , N-MePhe 4 , Gly-ol]-enkephalin (DAMGO) Tocris Biosciences Cat# 1171 SNC80 Tocris Biosciences Cat# 0764/10 (− )-U50488 Tocris Biosciences Cat# 0496/10 nociceptin Abcam Cat# ab120741 SNAP-Surface® Alexa Fluor® 488 New England Biolabs Cat# S9124S HaloTag® Alexa Fluor® 660 Promega Cat# G1001 ketamine Sigma Aldrich Cat# K113 xylazine Sigma Aldrich Cat# K113 isoflurane Zoetis UK Limited Cat# 50019100 Lumafluor Retrobeads Lumafluor Inc. N/A Carprofen (Rimadyl) Zoetis UK Limited N/A Amoxicillin (Betamox) Norbrook UK N/A Naloxone NIDA N/A SulfoCy5-buprenorphine University of Nottingham, Schembri et al. 27 N/A Lumi4-Tb Revvity Cat# SSNPTBD Tag-lite SNAP-CLIP labeling reagent (5x) Revvity Cat# LABMED Critical commercial assays Bradford protein assay Bio-Rad, Hercules, CA Cat# 5000001 Gibson HiFi assembly kit New England Biolabs Cat# E2621 Experimental models: cell lines HEK 293T (Female) ATCC (Virginia, USA) Cat# CRL-3216 Flp-InTM-CHO Cell Line InvitrogenTM/ThermoFisher Scientific Cat# R75807 Experimental models: organisms/strains B6.Cg-7630403G23Rik Tg(Th− cre)1Tmd /J TH-Cre1 mice Jackson Laboratory, Bar Harbor, Maine Strain #008601 RRID:IMSR_JAX:008601 Recombinant DNA WT-Gαi 2 cDNA Resource Center, cDNA.org Cat# GNAI200000 masGRKct-Rluc8 Nevin Lambert, University of Georgia. ..

    Modification:

    Article Title: Targeted inhibition of mu-opioid receptors in neuronal subpopulations by membrane-tethered Naloxo-DART antagonists.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9-pDISPLAY-Halo-LK3-TM Virovek, this paper N/A pAAV9-Ef1a-DIO-EYFP Gift from Karl Deisseroth Addgene viral prep Cat# 27056-AAV9 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 11965092 Fetal Bovine Serum (FBS) Gibco Cat# A5256701 Earle’s balanced salt solution (EBSS) Life Technologies Cat# 14155063 Polyethylenimine (PEI) PolySciences Inc Cat# 23966 Penicillin-Streptomycin Gibco Cat# 15140122 D-PBS Gibco Cat# 14190144 DAMGO, [ 3 H] Novandi Chemistry AB Cat# NT1077 coelenterazine h NanoLight Cat# 301 Poly-D-Lysine hydrobromide Sigma Aldrich Cat# P6407 Nano-Glo luciferase assay system (Furimizine) Promega Cat# N1130 [D-Ala 2 , N-MePhe 4 , Gly-ol]-enkephalin (DAMGO) Tocris Biosciences Cat# 1171 SNC80 Tocris Biosciences Cat# 0764/10 (− )-U50488 Tocris Biosciences Cat# 0496/10 nociceptin Abcam Cat# ab120741 SNAP-Surface® Alexa Fluor® 488 New England Biolabs Cat# S9124S HaloTag® Alexa Fluor® 660 Promega Cat# G1001 ketamine Sigma Aldrich Cat# K113 xylazine Sigma Aldrich Cat# K113 isoflurane Zoetis UK Limited Cat# 50019100 Lumafluor Retrobeads Lumafluor Inc. N/A Carprofen (Rimadyl) Zoetis UK Limited N/A Amoxicillin (Betamox) Norbrook UK N/A Naloxone NIDA N/A SulfoCy5-buprenorphine University of Nottingham, Schembri et al. 27 N/A Lumi4-Tb Revvity Cat# SSNPTBD Tag-lite SNAP-CLIP labeling reagent (5x) Revvity Cat# LABMED Critical commercial assays Bradford protein assay Bio-Rad, Hercules, CA Cat# 5000001 Gibson HiFi assembly kit New England Biolabs Cat# E2621 Experimental models: cell lines HEK 293T (Female) ATCC (Virginia, USA) Cat# CRL-3216 Flp-InTM-CHO Cell Line InvitrogenTM/ThermoFisher Scientific Cat# R75807 Experimental models: organisms/strains B6.Cg-7630403G23Rik Tg(Th− cre)1Tmd /J TH-Cre1 mice Jackson Laboratory, Bar Harbor, Maine Strain #008601 RRID:IMSR_JAX:008601 Recombinant DNA WT-Gαi 2 cDNA Resource Center, cDNA.org Cat# GNAI200000 masGRKct-Rluc8 Nevin Lambert, University of Georgia. ..

    Luciferase:

    Article Title: Targeted inhibition of mu-opioid receptors in neuronal subpopulations by membrane-tethered Naloxo-DART antagonists.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9-pDISPLAY-Halo-LK3-TM Virovek, this paper N/A pAAV9-Ef1a-DIO-EYFP Gift from Karl Deisseroth Addgene viral prep Cat# 27056-AAV9 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 11965092 Fetal Bovine Serum (FBS) Gibco Cat# A5256701 Earle’s balanced salt solution (EBSS) Life Technologies Cat# 14155063 Polyethylenimine (PEI) PolySciences Inc Cat# 23966 Penicillin-Streptomycin Gibco Cat# 15140122 D-PBS Gibco Cat# 14190144 DAMGO, [ 3 H] Novandi Chemistry AB Cat# NT1077 coelenterazine h NanoLight Cat# 301 Poly-D-Lysine hydrobromide Sigma Aldrich Cat# P6407 Nano-Glo luciferase assay system (Furimizine) Promega Cat# N1130 [D-Ala 2 , N-MePhe 4 , Gly-ol]-enkephalin (DAMGO) Tocris Biosciences Cat# 1171 SNC80 Tocris Biosciences Cat# 0764/10 (− )-U50488 Tocris Biosciences Cat# 0496/10 nociceptin Abcam Cat# ab120741 SNAP-Surface® Alexa Fluor® 488 New England Biolabs Cat# S9124S HaloTag® Alexa Fluor® 660 Promega Cat# G1001 ketamine Sigma Aldrich Cat# K113 xylazine Sigma Aldrich Cat# K113 isoflurane Zoetis UK Limited Cat# 50019100 Lumafluor Retrobeads Lumafluor Inc. N/A Carprofen (Rimadyl) Zoetis UK Limited N/A Amoxicillin (Betamox) Norbrook UK N/A Naloxone NIDA N/A SulfoCy5-buprenorphine University of Nottingham, Schembri et al. 27 N/A Lumi4-Tb Revvity Cat# SSNPTBD Tag-lite SNAP-CLIP labeling reagent (5x) Revvity Cat# LABMED Critical commercial assays Bradford protein assay Bio-Rad, Hercules, CA Cat# 5000001 Gibson HiFi assembly kit New England Biolabs Cat# E2621 Experimental models: cell lines HEK 293T (Female) ATCC (Virginia, USA) Cat# CRL-3216 Flp-InTM-CHO Cell Line InvitrogenTM/ThermoFisher Scientific Cat# R75807 Experimental models: organisms/strains B6.Cg-7630403G23Rik Tg(Th− cre)1Tmd /J TH-Cre1 mice Jackson Laboratory, Bar Harbor, Maine Strain #008601 RRID:IMSR_JAX:008601 Recombinant DNA WT-Gαi 2 cDNA Resource Center, cDNA.org Cat# GNAI200000 masGRKct-Rluc8 Nevin Lambert, University of Georgia. ..

    Labeling:

    Article Title: Targeted inhibition of mu-opioid receptors in neuronal subpopulations by membrane-tethered Naloxo-DART antagonists.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9-pDISPLAY-Halo-LK3-TM Virovek, this paper N/A pAAV9-Ef1a-DIO-EYFP Gift from Karl Deisseroth Addgene viral prep Cat# 27056-AAV9 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 11965092 Fetal Bovine Serum (FBS) Gibco Cat# A5256701 Earle’s balanced salt solution (EBSS) Life Technologies Cat# 14155063 Polyethylenimine (PEI) PolySciences Inc Cat# 23966 Penicillin-Streptomycin Gibco Cat# 15140122 D-PBS Gibco Cat# 14190144 DAMGO, [ 3 H] Novandi Chemistry AB Cat# NT1077 coelenterazine h NanoLight Cat# 301 Poly-D-Lysine hydrobromide Sigma Aldrich Cat# P6407 Nano-Glo luciferase assay system (Furimizine) Promega Cat# N1130 [D-Ala 2 , N-MePhe 4 , Gly-ol]-enkephalin (DAMGO) Tocris Biosciences Cat# 1171 SNC80 Tocris Biosciences Cat# 0764/10 (− )-U50488 Tocris Biosciences Cat# 0496/10 nociceptin Abcam Cat# ab120741 SNAP-Surface® Alexa Fluor® 488 New England Biolabs Cat# S9124S HaloTag® Alexa Fluor® 660 Promega Cat# G1001 ketamine Sigma Aldrich Cat# K113 xylazine Sigma Aldrich Cat# K113 isoflurane Zoetis UK Limited Cat# 50019100 Lumafluor Retrobeads Lumafluor Inc. N/A Carprofen (Rimadyl) Zoetis UK Limited N/A Amoxicillin (Betamox) Norbrook UK N/A Naloxone NIDA N/A SulfoCy5-buprenorphine University of Nottingham, Schembri et al. 27 N/A Lumi4-Tb Revvity Cat# SSNPTBD Tag-lite SNAP-CLIP labeling reagent (5x) Revvity Cat# LABMED Critical commercial assays Bradford protein assay Bio-Rad, Hercules, CA Cat# 5000001 Gibson HiFi assembly kit New England Biolabs Cat# E2621 Experimental models: cell lines HEK 293T (Female) ATCC (Virginia, USA) Cat# CRL-3216 Flp-InTM-CHO Cell Line InvitrogenTM/ThermoFisher Scientific Cat# R75807 Experimental models: organisms/strains B6.Cg-7630403G23Rik Tg(Th− cre)1Tmd /J TH-Cre1 mice Jackson Laboratory, Bar Harbor, Maine Strain #008601 RRID:IMSR_JAX:008601 Recombinant DNA WT-Gαi 2 cDNA Resource Center, cDNA.org Cat# GNAI200000 masGRKct-Rluc8 Nevin Lambert, University of Georgia. ..

    Bradford Protein Assay:

    Article Title: Targeted inhibition of mu-opioid receptors in neuronal subpopulations by membrane-tethered Naloxo-DART antagonists.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9-pDISPLAY-Halo-LK3-TM Virovek, this paper N/A pAAV9-Ef1a-DIO-EYFP Gift from Karl Deisseroth Addgene viral prep Cat# 27056-AAV9 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 11965092 Fetal Bovine Serum (FBS) Gibco Cat# A5256701 Earle’s balanced salt solution (EBSS) Life Technologies Cat# 14155063 Polyethylenimine (PEI) PolySciences Inc Cat# 23966 Penicillin-Streptomycin Gibco Cat# 15140122 D-PBS Gibco Cat# 14190144 DAMGO, [ 3 H] Novandi Chemistry AB Cat# NT1077 coelenterazine h NanoLight Cat# 301 Poly-D-Lysine hydrobromide Sigma Aldrich Cat# P6407 Nano-Glo luciferase assay system (Furimizine) Promega Cat# N1130 [D-Ala 2 , N-MePhe 4 , Gly-ol]-enkephalin (DAMGO) Tocris Biosciences Cat# 1171 SNC80 Tocris Biosciences Cat# 0764/10 (− )-U50488 Tocris Biosciences Cat# 0496/10 nociceptin Abcam Cat# ab120741 SNAP-Surface® Alexa Fluor® 488 New England Biolabs Cat# S9124S HaloTag® Alexa Fluor® 660 Promega Cat# G1001 ketamine Sigma Aldrich Cat# K113 xylazine Sigma Aldrich Cat# K113 isoflurane Zoetis UK Limited Cat# 50019100 Lumafluor Retrobeads Lumafluor Inc. N/A Carprofen (Rimadyl) Zoetis UK Limited N/A Amoxicillin (Betamox) Norbrook UK N/A Naloxone NIDA N/A SulfoCy5-buprenorphine University of Nottingham, Schembri et al. 27 N/A Lumi4-Tb Revvity Cat# SSNPTBD Tag-lite SNAP-CLIP labeling reagent (5x) Revvity Cat# LABMED Critical commercial assays Bradford protein assay Bio-Rad, Hercules, CA Cat# 5000001 Gibson HiFi assembly kit New England Biolabs Cat# E2621 Experimental models: cell lines HEK 293T (Female) ATCC (Virginia, USA) Cat# CRL-3216 Flp-InTM-CHO Cell Line InvitrogenTM/ThermoFisher Scientific Cat# R75807 Experimental models: organisms/strains B6.Cg-7630403G23Rik Tg(Th− cre)1Tmd /J TH-Cre1 mice Jackson Laboratory, Bar Harbor, Maine Strain #008601 RRID:IMSR_JAX:008601 Recombinant DNA WT-Gαi 2 cDNA Resource Center, cDNA.org Cat# GNAI200000 masGRKct-Rluc8 Nevin Lambert, University of Georgia. ..



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